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GAM 151 and GSS 140 Gas Metabolism Monitoring System Classical respirometry measurements can be made while the subjects are in whole body chambers. The concentrations of O2 and CO2 entering the chambers are compared to the concentrations leaving the chambers. From these values, and a measure of the flow through each chamber, we can derive the rate of oxygen consumption, and the rate of CO2 production, for each subject. This allows calculation of the respiratory quotient, and the rate oxygen metabolism, calculated using Weir's equation.
The unique design of the EMMS respirometry system allows multiple chambers to be monitored with a single gas analyser. Each chamber is sampled in turn for up to 2 minutes (user selectable), using a technique called multiplexing. The outputs from each chamber enter a special manifold, which selects one of the chamber outputs and routes the gas into the analyser for measurement. Special drier tubes ensure that the sampling gases are completely free of moisture before they are sampled. This minimises any errors which would otherwise be present in the measurement system. The concentrations of O2, CO2 and the flow rate, are all measured to a high degree of accuracy. The system supports up to 4 whole-body plethysmographs (PLY 31x) as standard, each containing an animal. A bias flow air supply (AIR 140) pulls a constant source of fresh air through each plethysmograph. The gas multiplexer (GSS 140) diverts the air from one of the plethysmographs (or the reference line) to the GAM 151 gas analyser, which measures the CO2 and O2 concentrations.
Click on screen shot for larger image Parameters Measured
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